Journal: Nature Communications
Article Title: Dual asparagine-depriving nanoparticles against solid tumors
doi: 10.1038/s41467-025-60798-y
Figure Lengend Snippet: a In vivo fluorescence imaging of tumor-bearing mice 24 h after intravenous injection of M Cy5.5 As and M Cy5.5 AHAs ( n = 3 mice). The unit of the scale bar is ×10 9 [p/s/cm 2 /sr]/[µW/cm 2 ]. b Left: Confocal fluorescence imaging of tumor sections after administration of M Cy5.5 As and M Cy5.5 AHAs. The 4T1 tumor cells and immune cells were indicated as CD44 and CD45 positive cells (red fluorescence), respectively. M Cy5.5 As and M Cy5.5 AHAs were pseudo-colored with green fluorescence. DAPI (blue fluorescence) was used to label the nuclei. Scale bars: 15 µm. Right: Values of Pearson’s correlation coefficient between Cy5.5-incorporated NPs and 4T1 tumor cells or immune cells. Data are represented as mean ± SD ( n = 10 independent samples). P values were calculated using an unpaired two-tailed Student’s t test. c Average tumor growth curves. Data are represented as mean ± SD ( n = 6 mice). P values were calculated using a two-way ANOVA followed by Tukey’s multiple comparisons test. d Survival curves of the mice receiving indicated treatment ( n = 6 mice). P values were calculated using a log-rank (Mantel–Cox) test. e The Asn levels in tumor cells isolated from tumors on day 26 after indicated treatment. Data are represented as mean ± SD ( n = 3 independent samples). P values were calculated using a one-way ANOVA followed by Tukey’s post-hoc test. f Heatmap of mRNA levels of ASNS , PSAT1 , GPT2 , MTHFD2 , and SLC1A5 in tumor cells isolated from tumors after indicated treatment. g Left: representative H&E-stained sections of lungs from indicated groups. Metastatic tumors are indicated with black dotted circles. Scale bars: 100 μm. See also Supplementary Fig. for complete data. Right: the number of tumor foci in lungs after indicated treatment. Data are represented as mean ± SD ( n = 3 mice). P values were calculated using a one-way ANOVA followed by Tukey’s post-hoc test. h , i Expression levels of N-cadherin ( h ) and E-cadherin ( i ) in tumors after indicated treatments. Data are represented as mean ± SD ( n = 3 independent samples). P values were calculated using a one-way ANOVA followed by Tukey’s post-hoc test. Asn asparagine, Cy5.5 cyanine 5.5, Rot rotenone, ASNase L -asparaginase. Source data are provided as a Source Data file.
Article Snippet: Tumor sections were stained with either anti-CD44 rabbit polyclonal antibody (Servicebio, GB112054 ) or anti-CD45 rabbit polyclonal antibody (Servicebio, GB113885 ), and visualized using CLSM.
Techniques: In Vivo, Fluorescence, Imaging, Injection, Two Tailed Test, Isolation, Staining, Expressing
Proteintech is a verified supplier 
![a In vivo fluorescence imaging of tumor-bearing mice 24 h after intravenous injection of M Cy5.5 As and M Cy5.5 AHAs ( n = 3 mice). The unit of the scale bar is ×10 9 [p/s/cm 2 /sr]/[µW/cm 2 ]. b Left: Confocal fluorescence imaging of tumor sections after administration of M Cy5.5 As and M Cy5.5 AHAs. The 4T1 tumor cells and immune cells were indicated as CD44 and <t>CD45</t> <t>positive</t> cells (red fluorescence), respectively. M Cy5.5 As and M Cy5.5 AHAs were pseudo-colored with green fluorescence. DAPI (blue fluorescence) was used to label the nuclei. Scale bars: 15 µm. Right: Values of Pearson’s correlation coefficient between Cy5.5-incorporated NPs and 4T1 tumor cells or immune cells. Data are represented as mean ± SD ( n = 10 independent samples). P values were calculated using an unpaired two-tailed Student’s t test. c Average tumor growth curves. Data are represented as mean ± SD ( n = 6 mice). P values were calculated using a two-way ANOVA followed by Tukey’s multiple comparisons test. d Survival curves of the mice receiving indicated treatment ( n = 6 mice). P values were calculated using a log-rank (Mantel–Cox) test. e The Asn levels in tumor cells isolated from tumors on day 26 after indicated treatment. Data are represented as mean ± SD ( n = 3 independent samples). P values were calculated using a one-way ANOVA followed by Tukey’s post-hoc test. f Heatmap of mRNA levels of ASNS , PSAT1 , GPT2 , MTHFD2 , and SLC1A5 in tumor cells isolated from tumors after indicated treatment. g Left: representative H&E-stained sections of lungs from indicated groups. Metastatic tumors are indicated with black dotted circles. Scale bars: 100 μm. See also Supplementary Fig. for complete data. Right: the number of tumor foci in lungs after indicated treatment. Data are represented as mean ± SD ( n = 3 mice). P values were calculated using a one-way ANOVA followed by Tukey’s post-hoc test. h , i Expression levels of N-cadherin ( h ) and E-cadherin ( i ) in tumors after indicated treatments. Data are represented as mean ± SD ( n = 3 independent samples). P values were calculated using a one-way ANOVA followed by Tukey’s post-hoc test. Asn asparagine, Cy5.5 cyanine 5.5, Rot rotenone, ASNase L -asparaginase. Source data are provided as a Source Data file.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5482/pmc12215482/pmc12215482__41467_2025_60798_Fig4_HTML.jpg)
